Blood—Brain Barrier Genomics and Cloning of a Novel Organic Anion Transporter
نویسندگان
چکیده
منابع مشابه
Expression cloning and characterization of a novel multispecific organic anion transporter.
Numerous drugs and endogenous compounds are efficiently excreted from the renal proximal tubule via carrier-mediated pathways. Transepithelial excretion of organic anions occurs via their accumulative transport from the blood into the proximal tubule cells across the basolateral membrane and subsequent secretion into the urine through the apical membrane. Here we report on the isolation of a no...
متن کاملMolecular cloning and characterization of a new multispecific organic anion transporter from rat brain.
A cDNA encoding the new member of the multispecific organic anion transporter family, OAT3, was isolated by the reverse transcription-polymerase chain reaction cloning method. Degenerate primers were designed based on the sequences conserved among OAT1, OAT2, and organic cation transporter 1 (OCT1), and reverse transcription-polymerase chain reaction was performed using rat brain poly(A)+ RNA. ...
متن کاملMolecular cloning and functional expression of a multispecific organic anion transporter from human kidney.
Recently, we isolated the multispecific organic anion transporter (OAT1) from the rat kidney, which plays important roles in the renal elimination of endogenous and exogenous organic anions including clinically important drugs. In the present study, we cloned and characterized human OAT1. Two cDNA clones, hOAT1-1 cDNA and hOAT1-2 cDNA, were isolated from a human kidney cDNA library, whose amino...
متن کاملCloning and functional characterization of a new multispecific organic anion transporter, OAT-K2, in rat kidney.
We have isolated a cDNA coding a new organic anion transporter, OAT-K2, expressed specifically in rat kidney. The OAT-K2 cDNA had an open reading frame encoding a 498-amino acid protein (calculated molecular mass of 55 kDa) that shows 91% identity with the rat kidney-specific organic anion transporter, OAT-K1. Reverse transcription-coupled polymerase chain reaction analyses revealed that the OA...
متن کاملMolecular cloning and functional characterization of a polyspecific organic anion transporter from Caenorhabditis elegans.
We have cloned a polyspecific organic anion transporter from Caenorhabditis elegans and elucidated its functional characteristics. The C. elegans anion transporter (CeOAT1) codes for a protein of 526 amino acids containing 12 putative transmembrane domains. It exhibits significant homology at the level of amino acid sequence to the C. elegans organic cation transporter and to the mammalian orga...
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ژورنال
عنوان ژورنال: Journal of Cerebral Blood Flow & Metabolism
سال: 2007
ISSN: 0271-678X,1559-7016
DOI: 10.1038/sj.jcbfm.9600538